horse radish peroxidase conjugated immpress anti mouse igg  (Vector Laboratories)

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

Article Snippet: The anti-mCII ELISA kits were used according to manufacturer's protocol (2036T; Chondrex).

Techniques: Indirect ELISA

Journal: Bioactive Materials

Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

doi: 10.1016/j.bioactmat.2026.03.025

Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: Integrative Medicine Research

Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS

doi: 10.1016/j.imr.2025.101264

Figure Lengend Snippet: Pg-hWE alleviates PCOS symptoms in a mouse model based on H&E staining. (A) PCOS mouse model construction. The mice were subcutaneously administered with 30 mg/kg of DHEA for six weeks. One week prior to start of SC administration, each group was administered the appropriate drug (tap water, dextrin, and Pg-hWE) orally. (B) Body weight was measured once a week. (C) Results of the AMH ELISA with mouse serum. (D) Mouse ovary histology by H&E staining. (E) Results of ovarian follicles by type. # p < 0.05 ## p < 0.01 compared with the Normal group, * p < 0.05 and ⁎⁎ p < 0.01 compared with the PCOS group.

Article Snippet: ELISA of mouse serum was performed using the CUSABIO's anti-Müllerian hormone ELISA kit (CUSABIO, Houston, TX, USA) as reported previously, and all preparation and experimental procedures were performed according to the protocol provided by CUSABIO.

Techniques: Staining, Enzyme-linked Immunosorbent Assay